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Nitrogen (N) transformation in soils is crucial in determining N availability for plant growth. Row crop producers have widely adopted cover crops across the U.S. However, there is limited knowledge about N transformations in commercial fields with and without cover crops. Furthermore, there is lack of understanding about the spatial variability of potential N mineralization in row crops and how it varies within a field and between cropping systems. An in-situ mineralization study was conducted in two Alabama row crop farms to evaluate the variability of potential N mineralization across locations and within a farm. The results revealed the variability in N mineralization within farms at both the locations. It was reported that a farm with cover crop and residue retention history had a mineralization rate of 1.18 to 3.89 lb/acre/day. In contrast, another farm with no cover crop had a mineralization potential of 0.93 to 1.17 lb/acre/day. These findings underscore the importance of cover crops and residue retention for enhancing N mineralization potential.